Western Blot:Article Title: Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ABfloTM 555-conjugated Goat Anti-Mouse IgG (H + L) Abclonal Cat# AS057; RRID: AB_2768321 Alexa Fluor 647-conjugated Goat AntiRabbit IgG (H + L) Abclonal Cat# AS060; RRID: AB_2768330 Fluorescein (FITC)–conjugated Goat AntiRat IgG(H + L) Proteintech Cat# SA00003-11; RRID: AB_2890989 Biological samples Human nondiabetic wound tissue samples the First Affiliated Hospital of Sun Yat-sen University N/A Human diabetic wound tissue samples the First Affiliated Hospital of Sun Yat-sen University N/A Chemicals, peptides, and recombinant proteins LPS Sigma Aldrich Cat# L2880 Recombinant Murine IL-4 Peprotech Cat# 214-14 Phenylpyruvate Sigma Aldrich Cat# 286958 Phenylalanine MedChemExpress Cat# HY-N0215 Lipofectamine 2000 Transfection Reagent Invitrogen Cat# 11668019 Lipofectamine RNAi Max Transfection Reagent Invitrogen Cat# 13778030 Penicillin‒streptomycin Sigma Aldrich Cat# V900929 Fetal bovine serum ThermoFisher Scientific Cat# 10099-141 MCC950 Selleck Cat# S8930 ATP Selleck Cat# S5260 Recombinant M-CSF PeproTech Cat# 315-02 Red cell lysis buffer Beyotime Cat# C3702 Palmitic acid MedChemExpress Cat# HY-N0830 DMEM ThermoFisher Scientific Cat# C11995500BT DL-dithiothreitol MedChemExpress Cat# HY-15917 4-methylumbelliferyl-6-thio-bd-glucopyranoside Medbio Cat# MED20989 b-glucosidase Sigma Aldrich Cat# G4511 Critical commercial assays IP-ABE Palmitoylation Kit AIMS Cat# AM101314 HiScript 1st Strand cDNA Synthesis Kit Vazyme, China Cat# R111 Real-time Fluorescent Quantitative PCR Kit Vazyme, China Cat# Q321 ECL detection reagent EpiZyme Cat# SQ202 BCA assay kit Thermo Fisher Scientific Cat# 23225 IL-1b ELISA KIT MEIMIAN Cat# MM-0040M2 IL-18 ELISA KIT MEIMIAN Cat# MM-0169M2 TNF-a ELISA KIT MEIMIAN Cat# MM-0132M2 Deposited data LC-MS raw data files https://www.ebi.ac.uk/metabolights/ MTBLS7853 See Table S3 for LiP-SMap determination of PPA-bound proteins This paper N/A Experimental models: Cell lines Murine BMDM This paper N/A Mouse embryonic fibroblasts This paper N/A Experimental models: Organisms/strains C57BL/6J mice GemPharmatech N/A BKS-DB mice GemPharmatech N/A (Continued on next page) Cell Reports Medicine 4, 101129, August 15, 2023 e2
Immunohistochemistry:Article Title: Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ABfloTM 555-conjugated Goat Anti-Mouse IgG (H + L) Abclonal Cat# AS057; RRID: AB_2768321 Alexa Fluor 647-conjugated Goat AntiRabbit IgG (H + L) Abclonal Cat# AS060; RRID: AB_2768330 Fluorescein (FITC)–conjugated Goat AntiRat IgG(H + L) Proteintech Cat# SA00003-11; RRID: AB_2890989 Biological samples Human nondiabetic wound tissue samples the First Affiliated Hospital of Sun Yat-sen University N/A Human diabetic wound tissue samples the First Affiliated Hospital of Sun Yat-sen University N/A Chemicals, peptides, and recombinant proteins LPS Sigma Aldrich Cat# L2880 Recombinant Murine IL-4 Peprotech Cat# 214-14 Phenylpyruvate Sigma Aldrich Cat# 286958 Phenylalanine MedChemExpress Cat# HY-N0215 Lipofectamine 2000 Transfection Reagent Invitrogen Cat# 11668019 Lipofectamine RNAi Max Transfection Reagent Invitrogen Cat# 13778030 Penicillin‒streptomycin Sigma Aldrich Cat# V900929 Fetal bovine serum ThermoFisher Scientific Cat# 10099-141 MCC950 Selleck Cat# S8930 ATP Selleck Cat# S5260 Recombinant M-CSF PeproTech Cat# 315-02 Red cell lysis buffer Beyotime Cat# C3702 Palmitic acid MedChemExpress Cat# HY-N0830 DMEM ThermoFisher Scientific Cat# C11995500BT DL-dithiothreitol MedChemExpress Cat# HY-15917 4-methylumbelliferyl-6-thio-bd-glucopyranoside Medbio Cat# MED20989 b-glucosidase Sigma Aldrich Cat# G4511 Critical commercial assays IP-ABE Palmitoylation Kit AIMS Cat# AM101314 HiScript 1st Strand cDNA Synthesis Kit Vazyme, China Cat# R111 Real-time Fluorescent Quantitative PCR Kit Vazyme, China Cat# Q321 ECL detection reagent EpiZyme Cat# SQ202 BCA assay kit Thermo Fisher Scientific Cat# 23225 IL-1b ELISA KIT MEIMIAN Cat# MM-0040M2 IL-18 ELISA KIT MEIMIAN Cat# MM-0169M2 TNF-a ELISA KIT MEIMIAN Cat# MM-0132M2 Deposited data LC-MS raw data files https://www.ebi.ac.uk/metabolights/ MTBLS7853 See Table S3 for LiP-SMap determination of PPA-bound proteins This paper N/A Experimental models: Cell lines Murine BMDM This paper N/A Mouse embryonic fibroblasts This paper N/A Experimental models: Organisms/strains C57BL/6J mice GemPharmatech N/A BKS-DB mice GemPharmatech N/A (Continued on next page) Cell Reports Medicine 4, 101129, August 15, 2023 e2
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